Neuroscience 2004 Abstract
| Presentation Number: | 693.1 |
|---|---|
| Abstract Title: | Demonstration of specific transynaptic transport in the visual system of adult Fisher rats using MnCl<sub>2</sub> as an MRI contrast agent. |
| Authors: |
Sandvig, A.*1
; Thuen, M.2
; Pedersen, T. B.2
; Sandvig, Y.1
; Haraldseth, O.2
; Brekken, C.2
; Berry, M.3
1Lab. of Regenerative Neurobiology, Inst. for Exptl. Med. Res., Ullevål Univ. Hosp., Oslo, Norway 2Norway, 4th Fl, Surgical Bldg, Ullevål University Hospital, 0407 Oslo, Norway, 0407, 3Dept of Circulation and Med. Imaging, 4th Fl, Surgical Bldg, Ullevål University Hospital, 0407 Oslo, Norway, 0407, |
| Primary Theme and Topics |
Techniques in Neuroscience - Staining, tracing and imaging techniques |
| Session: |
693. Imaging Techniques: fMRI and Other Poster |
| Presentation Time: | Tuesday, October 26, 2004 8:00 AM-9:00 AM |
| Location: | San Diego Convention Center - Hall A-H, Board # FFF17 |
| Keywords: | synaptic, axonal transport, in vivo, MRI |
Introduction: Rapid progress in the development of real time MRI imaging of specific nerve projections in the CNS has been made in the last few years. Of particular interest has been the demonstration of MnCl2 as an MRI contrast agent enabling visualisation of specific axonal pathways in the nervous system. We report here for the first time in vivo transynaptic transport of MnCL2 in the visual system of rats demonstrated with MRI.
Materials and Methods: Adult inbred Fisher rats (n=4) were subjected to unilateral intravitreal injection of 2 ul of 100 mM MnCl2 with all procedures in accordance to national local authority regulations. MRI was performed at 2.35 T using a Bruker Biospec Avance DBX-100 (Bruker AG, Germany), with a 72 mm volume coil for transmission and an actively decoupled quadrature rat head surface coil for receive-only. Water-cooled BGA-12 (200 mT/m) gradients were used. The MRI protocol was according to Brekken et al. 2003.
Results: MRI performed 36 hours post-injection demonstrated specific contrast up-take in vivo in the visual system including transynaptic transport of MnCl2 to the visual cortex.
Conclusion: We have for the first time demonstrated transynaptic transport of MnCl2 in vivo in the visual system of adult rats with MRI. Future studies will demonstrate the feasibility of using MnCl2 as an MRI contrast agent in a clinical setting.
References:
Brekken, C. et al.: Abstract. I SMRM. Bordeaux. France June 29.-July 1. 2003.
.
Materials and Methods: Adult inbred Fisher rats (n=4) were subjected to unilateral intravitreal injection of 2 ul of 100 mM MnCl2 with all procedures in accordance to national local authority regulations. MRI was performed at 2.35 T using a Bruker Biospec Avance DBX-100 (Bruker AG, Germany), with a 72 mm volume coil for transmission and an actively decoupled quadrature rat head surface coil for receive-only. Water-cooled BGA-12 (200 mT/m) gradients were used. The MRI protocol was according to Brekken et al. 2003.
Results: MRI performed 36 hours post-injection demonstrated specific contrast up-take in vivo in the visual system including transynaptic transport of MnCl2 to the visual cortex.
Conclusion: We have for the first time demonstrated transynaptic transport of MnCl2 in vivo in the visual system of adult rats with MRI. Future studies will demonstrate the feasibility of using MnCl2 as an MRI contrast agent in a clinical setting.
References:
Brekken, C. et al.: Abstract. I SMRM. Bordeaux. France June 29.-July 1. 2003.
.
Supported by VIRUUS, Ullevål University Hospital, Oslo, Norway
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2004 Neuroscience Meeting Planner. San Diego, CA: Society for Neuroscience, 2004. Online.
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