Neuroscience 2002 Abstract
| Presentation Number: | 73.17 |
|---|---|
| Abstract Title: | Gender and genotype dependent enlargement of the lateral and third ventricles in Waved-1. |
| Authors: |
Koshibu, K.*1
; Ahrens, E. T.2
; Levitt, P.3
1Dept Neurobiology, Univ Pittsburgh, Pittsburgh, PA 2Pittsburgh NMR Ctr for Biomed Res & Dept Biological Sciences, Carnegie Mellon Univ, Pittsburgh, PA 3Kennedy Ctr Human Devel & Dept Pharmacology, Vanderbilt Univ, Nashville, TN |
| Primary Theme and Topics |
Autonomic, Limbic and Other Systems - Neuroendocrine -- Steroid/brain interactions |
| Secondary Theme and Topics | Techniques in Neuroscience<br />- Staining, tracing and imaging techniques |
| Session: |
73. Neuroendocrine: steroid/brain interactions I Poster |
| Presentation Time: | Sunday, November 3, 2002 8:00 AM-9:00 AM |
| Location: | Hall A2-B3 N-24 |
| Keywords: | MRI, anxiety, TGF-alpha, neuroendocrine |
Waved-1 (wa-1) is an autosomal recessive mutation that causes a gradual decrease in transforming growth factor alpha (TGFa) expression during postnatal development. Previous studies using section reconstruction revealed that wa-1 males (on a mixed genetic background) exhibit an enlargement of the lateral ventricles with an associated late-onset deficit in fear conditioning. To evaluate the ventricular and periventricular structures more precisely, high-field (11.7 Tesla) magnetic resonance imaging (MRI) was employed. Male and female adult +/+ and wa-1 on a pure C57Bl6/J background were perfusion fixed. Three-dimensional (3D) T2 and diffusion-weighted spin-echo images of the entire brain were acquired. The lateral and third ventricles, hippocampus, amygdala, and striatum were segmented in 3D from these data. The lateral and third ventricles were enlarged by ~85% and ~50%, respectively, in +/+ females when compared to +/+ males. In addition, both male and female wa-1 mice had enlarged lateral ventricles compared to +/+ controls (~50% and ~30%, respectively). Although there were no obvious differences in the total volume determined for the hippocampus, amygdala, and striatum, qualitative observations suggest potential differences in the 3D shape of these brain areas between +/+ and wa-1 mice. Quantitative analyses may provide insight into structure-function relationships related to the behavior deficits in wa-1 mice.
Supported by NIMH grant MH45507 (PL), NSF IGERT predoctoral grant (KK) and NCRR grant P41-RR03631 (EA).
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2002 Neuroscience Meeting Planner. Orlando, FL: Society for Neuroscience, 2002. Online.
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