Neuroscience 2005 Abstract
| Presentation Number: | 702.6 |
|---|---|
| Abstract Title: | Development of <i>in vivo</i> electron paramagnetic resonance imaging for mapping infarction area and oxygen distribution in ischemic stroke. |
| Authors: |
Shen, J.*1
; Liu, S.1
; Rosen, G. M.3
; Timmins, G. S.1
; Tong, Y.2
; Liu, K. J.1
1Col. of Pharmacy, Univ. of New Mexico, Albuquerque, NM 2China, 2502 Marble Ave NE, 87131, 3School of Chinese Medicine, 2502 Marble Ave NE, 87131, |
| Primary Theme and Topics |
Disorders of the Nervous System - Ischemia -- Human studies and animal models |
| Secondary Theme and Topics | Techniques in Neuroscience<br />- Staining, Tracing, and Imaging Techniques |
| Session: |
702. Hypoxia/Ischemia: Animal Models II Slide |
| Presentation Time: | Tuesday, November 15, 2005 2:15 PM-2:30 PM |
| Location: | Washington Convention Center - Room 150B |
| Keywords: | cerebral ischemia, tissue oxygenation, imaging, magnetic resonance |
Noninvasive brain imaging is much needed for the study of the pathophysiology of neurological disorders, such as stroke. Magnetic resonance imaging (MRI) provides high-resolution morphological images useful in diagnostic radiology to differentiate between normal and abnormal/pathological states in brains. However, there is a lack of noninvasive method to directly measure and map oxygen concentration in the brain, in vivo and in real time, allowing estimates of oxygen distribution. Here we have developed the electron paramagnetic resonance image (EPRI) technique to map oxygen distribution in the ischemic brain of mice. EPRI is a magnetic resonance technique to detect paramagnetic species such as free radicals. As oxygen can broad the linewidth of EPR spectra of nitroxides via spin-spin exchange, we can detect oxygen concentration by analyzing the changes of linewidth of the nitroxides. To map oxygen distribution in the brain, we used a newly synthesized BBB permeable nitroxides, acetoxymethyl ester of 3-carboxy-2,2,5,5-tetramethyl-1-pyrrolidinyloxy (AM-CTP). Focal cerebral ischemia in male c57 mice was induced by occlusion of middle cerebral artery (MCAO). By co-registration with MRI, EPRI was able to identify the ischemic core in the brain. Most importantly, we successfully obtained the first oxygen mapping in the brain of mice that were subjected to MCAO. The EPR image demonstrates that focal cerebral ischemia dramatically decreased tissue oxygenation in the ischemic region, with the ischemic core being more severely affected than the ischemic penumbra. The spatial resolution of the image is about 0.3 mm. With this newly acquired ability to achieve oxygen mapping in the ischemic brain of mice, EPRI will be a useful imaging technique that complements other techniques such as MRI and positron emission tomography in obtaining valuable functional/physiological images.
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2005 Neuroscience Meeting Planner. Washington, DC: Society for Neuroscience, 2005. Online.
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