Neuroscience 2003 Abstract
| Presentation Number: | 671.14 |
|---|---|
| Abstract Title: | Are newly generated granule cells in the lesioned adult and aged hippocampus capable of long-term survival? |
| Authors: |
Abdel-Rahman, A.*1,2
; Rao, M. S.1,2
; Stanley, D.1,2
; Shetty, A. K.1,2
1Dept. Surgery (Div. Neurosurgery), Duke Univ, Durham, NC 2NC, LSRC Bldg Sec C, 27710, |
| Primary Theme and Topics |
Development - Neurogenesis and Gliogenesis -- Neural stem cells |
| Secondary Theme and Topics | Neurological and Psychiatric Conditions<br />- Epilepsy<br />-- Human studies and animal models |
| Session: |
671. Adult Neurogenesis IV Poster |
| Presentation Time: | Tuesday, November 11, 2003 2:00 PM-3:00 PM |
| Location: | Morial Convention Center - Hall F-I, Board # A17 |
| Keywords: | STEM CELL, DENTATE GYRUS, NEUROGENESIS, EPILEPSY |
Neurogenesis occurs in the adult and the aged dentate gyrus (DG), and about 50% of newly generated neurons survive for prolonged periods in the adult DG suggesting that newly formed neurons play roles in learning and memory. Kainic acid (KA) induced hippocampal CA3-lesion, increases DG neurogenesis in the young hippocampus, and maintains DG neurogenesis to the level of age-matched intact hippocampus in the middle-aged and the aged hippocampus (Rao et al., SFN abstracts, 2003). However, it is unknown whether newly generated neurons in the DG of lesioned adult and aged hippocampus are capable of enduring survival. We quantified the long-term survival of new neurons in the DG of adult (4-months-old), middle-aged (12-months-old) and aged (24-months-old) F344 rats generated at early post-KA lesion. Newly generated neurons were labeled using daily injections of 5’-bromodeoxyuridine (BrdU; 100 mg/Kg bw) from 4-15 days post-lesion. At 6-months post-lesion, the survival of DG neurons generated during 4-15 days post-lesion were quantified using BrdU/NeuN immunostaining and the optical fractionator. In all age groups, the chronically injured hippocampus supported the survival of neurons generated during early post-lesion. Interestingly, the rate of survival was similar (50-60%) across the three age groups, in comparison to the respective number of new neurons identified with BrdU/doublecortin staining at 16 days post-lesion. Thus, a significant number of new granule cells generated during early post-injury period exhibit protracted survival, regardless of the age at the time of injury. This implies that the new granule cells in the injured adult and aged hippocampus contribute considerably to the aberrant mossy fiber synaptic re-organization, as the loss of granule cell targets persists in the KA-lesioned adult and aged hippocampus.
Supported by NIA Grant AG 20924 to A.K.S.
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2003 Neuroscience Meeting Planner. New Orleans, LA: Society for Neuroscience, 2003. Online.
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