Neuroscience 2005 Abstract
| Presentation Number: | 676.5 |
|---|---|
| Abstract Title: | A comparison of semiquantitative and fully quantitative [<sup>14</sup>C]-2-deoxyglucose autoradiography for the detection of PCP-induced metabolic hypofunction. |
| Authors: |
Cochran, S. M.*1,2
; Egerton, A.1,2
; Morris, B. J.1,3
; Pratt, J. A.1,2
1Yoshitomi Research Inst. of Neuroscience, Univ. of Strathclyde, Glasgow, United Kingdom 2Dept. Physiology & Pharmacology, Univ. of Strathclyde, Glasgow, United Kingdom 3United Kingdom, Yoshitomi Research Inst. of Neuroscience, G4 0NR, |
| Primary Theme and Topics |
Disorders of the Nervous System - Cognitive, Emotional and Behavioral State Disorders -- Schizophrenia: Animal models |
| Secondary Theme and Topics | Cognition and Behavior<br />- Animal Cognition and Behavior<br />-- Timing and temporal processing |
| Session: |
676. Schizophrenia: Glutamate Dysfunction Models II Poster |
| Presentation Time: | Tuesday, November 15, 2005 8:00 AM-9:00 AM |
| Location: | Washington Convention Center - Hall A-C, Board # TT69 |
| Keywords: | Schizophrenia, Attentional Flexibility, Prefrontal Cortex, Thalamus |
In the rat, glucose metabolism can be monitored using either fully quantitative [14-C]-2-deoxyglucose (2DG) autoradiography to assess local cerebral glucose utilisation (LCGU) or semiquantitative 2DG autoradiography to measure relative 2DG uptake. Previous studies within our laboratory have demonstrated that chronic intermittent PCP treatment induces both a metabolic hypofunction within schizophrenia-related brain regions using the fully quantitative technique (Cochran et al., 2003; Neuropsychopharm. 28:265-275), and specific deficits in the ability of rats to shift attentional set (Egerton et al., Program No. 796.13. 2004 Abstract Viewer/Itinerary Planner. Washington, DC: SFN). However, the fully quantitative technique is not compatible with behavioural testing, and hence limits direct association of the deficient attentional flexibility to the ineffective recruitment of the prefrontal cortex and associated circuitry. The aim of this study was to determine whether the end point measurement of 2DG autoradiography by these related methods are equivalent for the same treatment regime. If so, this would then enable monitoring of functional activity in parallel with behavioural task performance.
Adult male hooded Long Evans rats were treated with PCP (2.58mg/kg. i.p.) or vehicle intermittently over a 26 day period (n=11 per group). 72h after the final exposure to PCP, measurement of relative 2DG uptake was performed.
These data show that a PCP-induced reduction in metabolic activity in the prefrontal cortex and thalamic areas was demonstrable by both methods. The application of semiquantitative autoradiography alongside behavioural paradigms such as attentional set shifting will also be discussed.
Adult male hooded Long Evans rats were treated with PCP (2.58mg/kg. i.p.) or vehicle intermittently over a 26 day period (n=11 per group). 72h after the final exposure to PCP, measurement of relative 2DG uptake was performed.
These data show that a PCP-induced reduction in metabolic activity in the prefrontal cortex and thalamic areas was demonstrable by both methods. The application of semiquantitative autoradiography alongside behavioural paradigms such as attentional set shifting will also be discussed.
Supported by Mitsubishi Pharma Corporation, Japan
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2005 Neuroscience Meeting Planner. Washington, DC: Society for Neuroscience, 2005. Online.
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