Neuroscience 2005 Abstract
| Presentation Number: | 669.12 |
|---|---|
| Abstract Title: | Minocycline in a rat model of stroke: two-month follow-up study. |
| Authors: |
Ahtoniemi, T.*1
; Nurmi, A.2
; Puoliväli, J.2
; Pussinen, R.2
; Malm, T.1
; Jokivarsi, K.1
; Gröhn, O.1
; Koistinaho, J.1
; Koistinaho, M.2
; Yrjänheikki, J.2
1AIV Inst., Univ. Kuopio, Kuopio, Finland 2Finland, PO Box 1627, 70211, |
| Primary Theme and Topics |
Disorders of the Nervous System - Ischemia -- Neuroprotection and tolerance |
| Session: |
669. Ischemia: Experimental Therapeutics III Poster |
| Presentation Time: | Tuesday, November 15, 2005 11:00 AM-12:00 PM |
| Location: | Washington Convention Center - Hall A-C, Board # SS36 |
| Keywords: | ISCHEMIA, NEUROPROTECTION, BEHAVIOR |
Acute treatment with minocycline (MINO) reduces infarct size in focal cerebral ischemia. However, long term effects of MINO on ischemic brain damage and behavioral outcome have not been previously reported. The objective of this study was to explore the effects of acute MINO treatment on motor performance and cognitive functions during a 2-month follow-up after focal cerebral ischemia in male Sprague-Dawley rats.
Rats were subjected to 90 min middle cerebral artery occlusion (tMCAO) and treated with vehicle (Veh) or MINO starting 45 min after the occlusion and continued for 3 days. Total infarct volume was evaluated 14 days after tMCAO with T2- weighed magnetic resonance imaging (T2wt-MRI). Sensory-motor behavior and activity [neuroscore (NS), cylinder test (CT), Montoya’s staircase test (MT), beam traversing test (BT), open field (OF)] as well as cognitive functions [object recognition (OR), passive avoidance (PA)] were evaluated at different time points.
Significant post-ischemic functional deficits in NS, CT, and MT were observed in both Veh and MINO groups compared to sham-operated rats. Ischemic animals were also impaired in tests monitoring learning and memory and were hyperactive in OF. However, no significant improvement in functional or cognitive tests was observed after MINO treatment. The lack of beneficial effect of acute MINO treatment on behavior was supported by the T2wt-MRI, which revealed no significant differences in total infarct volume between groups (Veh 133±16 and MINO 116±17 mm3) at day 14 post-ischemia. These results indicate that 3-day treatment with MINO does not provide long-term neuroprotection and improvement in motor or cognitive functions after tMCAO. Importantly, the effect of long term sustained treatment regimen with MINO should be investigated in tMCAO.
Rats were subjected to 90 min middle cerebral artery occlusion (tMCAO) and treated with vehicle (Veh) or MINO starting 45 min after the occlusion and continued for 3 days. Total infarct volume was evaluated 14 days after tMCAO with T2- weighed magnetic resonance imaging (T2wt-MRI). Sensory-motor behavior and activity [neuroscore (NS), cylinder test (CT), Montoya’s staircase test (MT), beam traversing test (BT), open field (OF)] as well as cognitive functions [object recognition (OR), passive avoidance (PA)] were evaluated at different time points.
Significant post-ischemic functional deficits in NS, CT, and MT were observed in both Veh and MINO groups compared to sham-operated rats. Ischemic animals were also impaired in tests monitoring learning and memory and were hyperactive in OF. However, no significant improvement in functional or cognitive tests was observed after MINO treatment. The lack of beneficial effect of acute MINO treatment on behavior was supported by the T2wt-MRI, which revealed no significant differences in total infarct volume between groups (Veh 133±16 and MINO 116±17 mm3) at day 14 post-ischemia. These results indicate that 3-day treatment with MINO does not provide long-term neuroprotection and improvement in motor or cognitive functions after tMCAO. Importantly, the effect of long term sustained treatment regimen with MINO should be investigated in tMCAO.
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2005 Neuroscience Meeting Planner. Washington, DC: Society for Neuroscience, 2005. Online.
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