Neuroscience 2002 Abstract
| Presentation Number: | 737.5 |
|---|---|
| Abstract Title: | Cannabinoids modulate GABA release rom rat olfactory bulb. |
| Authors: |
Jaffe, E. H.*1
; Figueroa, L.1
1Lab Neuroquimica CBB, IVIC, Caracas 1020 A, Venezuela |
| Primary Theme and Topics |
Synaptic Transmission and Excitability - Neurotransmitters -- Cannabinoids |
| Secondary Theme and Topics | Synaptic Transmission and Excitability<br />- Neurotransmitters<br />-- GABA and glycine |
| Session: |
737. Neurotransmitters: cannabinoids I Poster |
| Presentation Time: | Wednesday, November 6, 2002 1:00 PM-2:00 PM |
| Location: | Hall A2-B3 C-48 |
| Keywords: | WIN55,212-2 |
Cannabinoids are novel neuromodulators derived from marijuana (Cannabis sativa) an old and widely used drug with important psychoactive effects. These substances have been described to act as retrograde messengers modulating the release of both glutamate and GABA at the level of the hippocampus and cerebellum. The cannabinoid receptor, CB1 is widely distributed in the CNS, with high density at the level of the inner layers of the olfactory bulb. In this structure GABA has been demonstrated to be highly concentrated by granule cells and we have previously shown that GABA is released in a Ca++ dependent manner when stimulated with KCl or glutamate.
We were interested to test whether Cannabinoids could modulate basal or stimulated GABA release. Slices containing the inner layers of the olfactory bulb were incubated with 3H-GABA and release measured using continuous superfusion. Slices were then exposed for 4 min with different concentrations of WIN55,212-2 an agonist of CB1 receptors. 1- and 5 uM of the drug, by its one was able to induce a modest but significant release of 3H-GABA. On the other hand the glutamate or KCL stimulates 3H-GABA release was not affected by exposing the slices with 5 uM of WIN55,212-2 for 20 min before and during stimulation.
Apparently at the level of the olfactory bulb Cannabinoids are able to enhance basal released GABA but under the present conditions, not the stimulated one.
We were interested to test whether Cannabinoids could modulate basal or stimulated GABA release. Slices containing the inner layers of the olfactory bulb were incubated with 3H-GABA and release measured using continuous superfusion. Slices were then exposed for 4 min with different concentrations of WIN55,212-2 an agonist of CB1 receptors. 1- and 5 uM of the drug, by its one was able to induce a modest but significant release of 3H-GABA. On the other hand the glutamate or KCL stimulates 3H-GABA release was not affected by exposing the slices with 5 uM of WIN55,212-2 for 20 min before and during stimulation.
Apparently at the level of the olfactory bulb Cannabinoids are able to enhance basal released GABA but under the present conditions, not the stimulated one.
Supported by E.H. jaffe
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2002 Neuroscience Meeting Planner. Orlando, FL: Society for Neuroscience, 2002. Online.
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