Neuroscience 2001 Abstract
| Presentation Number: | 718.6 |
|---|---|
| Abstract Title: | 5-HT<sub>2</sub> RECEPTOR STIMULATION CHANGES PROTEIN KINASE C EXPRESSION IN THE NEONATAL RAT SPINAL CORD. |
| Authors: |
MacDonald, S. C.*1
; Shay, B. L.1
; Sawchuk, M. A.1
; Hochman, S.1
; Buck, C. R.1
1Dept Physiol, Emory Univ Sch Med, Atlanta, GA |
| Primary Theme and Topics |
Sensory Systems - Pain -- Spinal cord processing |
| Secondary Theme and Topics | Motor Systems<br />- Reflex Function |
| Session: |
718. Pain modulation: GABA, glycine, serotonin and their receptors Poster |
| Presentation Time: | Wednesday, November 14, 2001 9:00 AM-10:00 AM |
| Location: | Exhibit Hall H-1 |
| Keywords: | Serotonin, Differential Display, Reflex, LTP |
5-HT2 receptor stimulation results in a long term potentiation in spinal reflex pathways (rLTP)(Machacek et al 1999 SFN). Novel gene expression is an essential component in maintaining LTP. Protein kinase C (PKC) ς and PKC γ isoforms are implicated in synaptic plasticity seen in hippocampal LTP. PKC γ is altered in spinal cord following injury. 5-HT2 receptors positively couple to PKC signaling pathways and the long term facilitatory effects of 5-HT may be due to upregulation and chronic activation of PKC in the spinal cord. It was our goal to see if changes in gene expression including PKC isoforms accompany 5-HT2 receptor-induced rLTP.
Neonatal rat spinal cords (P8-P11) were isolated, hemisected and maintained in vitro. Electrodes were used to record spinal reflexes. The 5-HT2 receptor agonist DOI was applied and changes in reflexes were recorded. Total RNA was harvested from cords after recording and cDNA was synthesized for PCR analysis of gene expression levels. Differential display PCR was used to identify differentially expressed genes. These identities are currently being confirmed. Changes in gene expression were studied using the iCycler real-time PCR system. DOI treatment of spinal cords yielded consistent reflex LTP. PKC γ was upregulated in all 3 DOI treated spinal cords while PKC ς was upregulated in 2/3. Immunohistochemistry is underway to localize PKC isoform expression changes within the spinal cord.
These results suggest that an increase in PKC expression accompanies 5-HT2 receptor-mediated rLTP. This may have relevance to mechanisms mediating spinal central sensitization.
Neonatal rat spinal cords (P8-P11) were isolated, hemisected and maintained in vitro. Electrodes were used to record spinal reflexes. The 5-HT2 receptor agonist DOI was applied and changes in reflexes were recorded. Total RNA was harvested from cords after recording and cDNA was synthesized for PCR analysis of gene expression levels. Differential display PCR was used to identify differentially expressed genes. These identities are currently being confirmed. Changes in gene expression were studied using the iCycler real-time PCR system. DOI treatment of spinal cords yielded consistent reflex LTP. PKC γ was upregulated in all 3 DOI treated spinal cords while PKC ς was upregulated in 2/3. Immunohistochemistry is underway to localize PKC isoform expression changes within the spinal cord.
These results suggest that an increase in PKC expression accompanies 5-HT2 receptor-mediated rLTP. This may have relevance to mechanisms mediating spinal central sensitization.
Supported by NINDS #NS40893-01 and SCM by Christopher Reeve Paralysis Foundation
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2001 Neuroscience Meeting Planner. San Diego, CA: Society for Neuroscience, 2001. Online.
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