Neuroscience 2004 Abstract
| Presentation Number: | 633.7 |
|---|---|
| Abstract Title: | Δ<sup>9</sup>-Tetrahydrocannabinol induces CREB phosphorylation in cerebellar granule cells. |
| Authors: |
Mongeau, R.*1
; Sanna, A.1
; Casu, M.1
; Pisu, C.1
; Congeddu, E.1
; Pani, L.1,2
1Neuroscienze Scarl, Cagliari, Italy 2Italy, Neurosci Scarl, Via Palabanda, 9, 09100, |
| Primary Theme and Topics |
Synaptic Transmission and Excitability - Intracellular Signaling Pathways -- Protein phosphorylation |
| Secondary Theme and Topics | Neurological and Psychiatric Conditions<br />- Addiction and Drugs of Abuse<br />-- Cannabinoids |
| Session: |
633. Protein Phosphorylation IV Poster |
| Presentation Time: | Tuesday, October 26, 2004 10:00 AM-11:00 AM |
| Location: | San Diego Convention Center - Hall A-H, Board # P23 |
| Keywords: | drug abuse, cannabinoids, SIGNAL TRANSDUCTION, GPCR |
Δ9-Tetrahydrocannabinol (Δ9-THC), the major psychoactive compound of marijuana, exerts direct cellular effects through the activation of G-protein-coupled CB1 and CB2 receptors located mainly in the brain and the immune system, respectively. The molecular mechanism by which cannabinoids alter gene expression in the brain is not well understood. The CB1 receptors coupled to Gi proteins activate both potassium channels and members of the mitogen-associated protein kinase family, and also inhibit adenylate cyclase and voltage-gated calcium channels. Moreover, there is evidence for an alternative CB1-mediated signalling pathway involving the activation of adenylate cyclase through stimulatory Gs proteins. The modulation of the phosphorylated cAMP-response element binding protein (p-CREB) by Δ9-THC was investigated in cerebellar granule cells, known to display an high distribution of CB1 receptors, to elucidate the cellular mechanism involved in CB1 receptors activation. Different concentrations (0, 0.3, 3, 30, 100, 300 nM) of Δ9-THC have been tested, and the relative amount of p-CREB was evaluated by Western blot. The results show that Δ9-THC 3 nM significantly increased the amount of p-CREB (+57% ± 14.75) compared to control. Further experiments will be carried out to investigate the signalling pathway involved in this effect of Δ9-THC.
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2004 Neuroscience Meeting Planner. San Diego, CA: Society for Neuroscience, 2004. Online.
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