Neuroscience 2004 Abstract
| Presentation Number: | 633.6 |
|---|---|
| Abstract Title: | Effect of Δ<sup>9</sup>-tetrahydrocannabinol on CREB phosphorylation in rat cerebellum: An immunohistochemical study. |
| Authors: |
Casu, M.*1
; Pisu, C.1
; Pinna Spada, G.1
; Tambaro, S.1
; Sanna, A.1
; Pani, L.1,2
1Morphology, Neurosci. S.C.A.R.L, Cagliari, Italy 2Italy, Via Palabanda 9, 09100, |
| Primary Theme and Topics |
Synaptic Transmission and Excitability - Intracellular Signaling Pathways -- Protein phosphorylation |
| Secondary Theme and Topics | Neurological and Psychiatric Conditions<br />- Addiction and Drugs of Abuse<br />-- Cannabinoids |
| Session: |
633. Protein Phosphorylation IV Poster |
| Presentation Time: | Tuesday, October 26, 2004 9:00 AM-10:00 AM |
| Location: | San Diego Convention Center - Hall A-H, Board # P23 |
| Keywords: | CANNABINOIDS, SIGNAL TRANSDUCTION, HISTOCHEMISTRY, DRUG ABUSE |
Numerous CNS processes, including neurotransmitter synthesis, gene expression, and cellular proliferation are controlled by neurotransmitters acting through second messenger systems that phosphorylate the transcription factor cyclic AMP response element-binding protein (CREB). Several converging lines of evidence indicate that drug of abuse may exert their profound long-term effects on the CNS by modulating signaling pathways controlling gene expression. Cannabinoids produce locomotor effects that include hypolocomotion, ataxia and catalepsy mediated by CB1 cannabinoids receptors located in motor control regions and cognitive impairment. Since there is evidence that cerebellum have a role not only in motor function but also in cognition, the purpose of the present study was to examine the effect of the Δ9-tetrahydrocannabinol (Δ9-THC), the principal psychoactive component of marijuana, on the phosphorylation of CREB in rat cerebellum, which present an high density of cannabinoids CB1 receptors and to determine which cell types in the cerebellum display this response to Δ9-THC. To this aim, we performed an immunohistochemistry study using an antibody raised against the phosphorylated form of CREB (p-CREB). The acute treatment with Δ9-THC at the dose of 2.5, 5 and 10 mg/kg induced a significant dose-dependent increase in the p-CREB in the granule cell layer of the cerebellum, with respect to controls. Following chronic Δ9-THC exposure at the dose of 10 mg/kg for 4 weeks, the induction of p-CREB was markedly attenuated compared to the respective vehicles.
These data provide evidence of the involvement of cerebellar circuits in the adaptive changes occurring during acute and chronic Δ9-THC exposure. It is plausible that this could be a mechanism by which Δ9-THC interfere with intended movements as well as cerebellar learning in general.
These data provide evidence of the involvement of cerebellar circuits in the adaptive changes occurring during acute and chronic Δ9-THC exposure. It is plausible that this could be a mechanism by which Δ9-THC interfere with intended movements as well as cerebellar learning in general.
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2004 Neuroscience Meeting Planner. San Diego, CA: Society for Neuroscience, 2004. Online.
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