Neuroscience 2002 Abstract
| Presentation Number: | 65.21 |
|---|---|
| Abstract Title: | 5-HT induced rhythmicity in the <i>in vitro</i> spinal cord of the neonatal mouse is partly dependent on D<sub>1</sub> and D<sub>2</sub> receptors. |
| Authors: |
Madriaga, M. A.*1,2
; McPhee, L. C.1,2
; Whelan, P. J.1,2
1Dept Physics & Biophysics, Univ Calgary, Calgary, Canada 2Neurosci. Res. Grp., Univ Calgary, Calgary, Canada |
| Primary Theme and Topics |
Motor Systems - Locomotion |
| Secondary Theme and Topics | Motor Systems<br />- Pattern Generation |
| Session: |
65. Locomotion: spinal processing Poster |
| Presentation Time: | Sunday, November 3, 2002 8:00 AM-9:00 AM |
| Location: | Hall A2-B3 K-6 |
| Keywords: | CPG, Locomotion, Mouse, 5-HT |
Serotonin plays an important role in locomotor pattern generation during early development. In the isolated in vitro rat and mouse spinal cord, bath applied 5-HT can evoke long-lasting rhythmic burst discharges from ventral roots. The 5-HT receptor subtypes that contribute to 5-HT evoked locomotion are considered to be 5-HT 1,2,7. However, a recent SFN abstract (Aduonum et al, 2001) showed that in some species of the neonatal rat 5-HT evoked locomotion persisted when all known 5-HT receptors were blocked. We tested the hypothesis that D1 and D2 receptors partly contribute to 5-HT (30 μM) induced locomotion using the isolated mouse (Swiss Webster) in vitro spinal cord as a model. Blockade of either D1 receptors (2 μM SCH-23390) or D2 receptors (10 μM Amisulpiride) did not completely block the locomotor-like discharge recorded from the L2 and L5 ventral roots (3/3 animals). However, bath application of both D1 and D2 antagonists did block the locomotor rhythm (4/4 animals). Blockade of 5-HT2 (100 nM ketanserin, 500 nM N-desmethylclozapine) receptor subtypes potently blocked the locomotor pattern. These results suggest that the locomotor pattern evoked by 5-HT in the neonatal mouse is mediated by a combination of 5-HT and dopaminergic receptors.
Supported by CIHR
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2002 Neuroscience Meeting Planner. Orlando, FL: Society for Neuroscience, 2002. Online.
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