Neuroscience 2004 Abstract
| Presentation Number: | 607.8 |
|---|---|
| Abstract Title: | Cannabinoid promotes the proliferation of neural stem/progenitor cells in the rat hippocampus both <I>in vitro</I> and <I>in vivo.</I> |
| Authors: |
Jiang, W.*1
; Van Cleemput, J. M.1
; Ji, S.1
; Zhang, X.1
1Neuropsychiat. Res. Unit, Dept. of Psychiatry, Univ. of Saskatchewan, Saskatoon, Canada |
| Primary Theme and Topics |
Development - Neurogenesis and Gliogenesis -- Neural stem cells |
| Secondary Theme and Topics | Development<br />- Neurogenesis and Gliogenesis<br />-- Proliferation |
| Session: |
607. Neurogenesis in the Dentate Gyrus II Poster |
| Presentation Time: | Tuesday, October 26, 2004 11:00 AM-12:00 PM |
| Location: | San Diego Convention Center - Hall A-H, Board # B15 |
| Keywords: | BRDU, DENTATE GYRUS, NEUROGENESIS, RAT |
Cannabis or marijuana has been used for recreational purposes and as a medicine for many centuries, but its addictive potential and some adverse side effects such as memory impairment, make it become the focus of strong social, legal, and medical controversy over its therapeutic utility. The settlement of this dispute may depend on the clarification of its mechanisms acting on brain. This study was undertaken to examine the effects of cannabinoids on the proliferation of neural stem/progenitor cells in the rat hippocampus both in vitro and in vivo. Using both WST-8 and bromodeoxyuridine (BrdU) ELISA methods, we found that the potent cannabinoid HU210 (10 nM - 500 nM) promoted significantly the proliferation of the cultured neural stem/progenitor cells collected from the rat hippocampus at E17. Using BrdU to label dividing cells in adult Long-Evens, Wistar and Fischer-344 rats, we further found that twice daily i.p. injections of the potent cannabinoid HU210 for 5 days increased significantly the number of BrdU-labeled cells in the dentate gyrus with the dose of 100 µg/kg but not with the dose of 25 µg/kg. In contrast, a single i.p. injection of the CB1 receptor antagonist AM281 reduced the number of BrdU-labeled cells in the dentate gyrus. We are now conducting experiments to examine the effects of cannabinoid on the differentiation of neural stem/progenitor cells.
Supported by postdoctoral fellowship awards from the College of Medicine, University of Saskatchewan to W.J. and from the Saskatchewan Health Research Foundation to S.P.J, and by a grant from the Canadian Institutes of Health Research (CIHR) awarded to X.Z.
Supported by postdoctoral fellowship awards from the College of Medicine, University of Saskatchewan to W.J. and from the Saskatchewan Health Research Foundation to S.P.J, and by a grant from the Canadian Institutes of Health Research (CIHR) awarded to X.Z.
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2004 Neuroscience Meeting Planner. San Diego, CA: Society for Neuroscience, 2004. Online.
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