Neuroscience 2003 Abstract
| Presentation Number: | 589.17 |
|---|---|
| Abstract Title: | Adrenergic and purinergic agonists alter norepinephrine transporter expression in rat dorsal root ganglia and celiac ganglia neurons <I>in vitro</I>. |
| Authors: |
Zheng, J.*1,2
; Dai, X.1
; Kreulen, D. L.1,2
1Dept. of Physiology, Michigan State Univ., East Lansing, MI 2Dept. of Neurol./Ophthalmology, Michigan State Univ., East Lansing, MI |
| Primary Theme and Topics |
Sensory Systems - Pain -- Neuropathic pain |
| Session: |
589. Therapeutics for Neuropathic Pain Poster |
| Presentation Time: | Tuesday, November 11, 2003 8:00 AM-9:00 AM |
| Location: | Morial Convention Center - Hall F-I, Board # F20 |
| Keywords: | SENSORY NEURONS , CELL CULTURE , RT-PCR, UPTAKE |
The sympathetic dependence of neuropathic pain is related to the upregulation of adrenergic receptors on primary afferent fibers and that these adrenergic receptors mediate the enhanced sensitivity of sensory fibers. We have found that norepinephrine transporter (NET) mRNA and protein of both dorsal root ganglia (DRG) and sympathetic ganglia are changed in L5/L6 spinal nerve ligation rats (Zheng et al, SFN 2002). To examine the relationships between sympathetic and sensory nerves, we evaluated the effect of sympathetic neurotransmitters on the expression and function of norepinephrine transporter in freshly dissociated DRG and celiac ganglia (CG) neurons. Freshly dissociated newborn rat DRG and CG neurons maintained in culture were incubated for 2 hr with the a1 agonist methoxamine, or the a2 agonist UK14304, or the purinergic agonist ab-methylene blue ATP. Real time PCR was used to analyze for NET mRNA and NET function was analyzed by measuring the uptake of the fluorescent probe ASP+ (4-(4-(dimethylamino)styryl)-N- methylpyridinium iodide (4-Di-1-ASP)), a NET substrate. Real time RT-PCR (2-(SDCT-CDCT)) demonstrated that the NET mRNA was increased 45 fold in UK14304 and methoxamine treated CG neurons. On the other hand, the NET mRNA was decreased or unchanged in DRG neurons by these adrenergic agonists. In both CG and DRG neurons NET mRNA was decreased in ATP treated neurons. ASP+ uptake was enhanced in DRG neurons incubated with UK14304. These results demonstrate that adrenergic and purinergic agonists can alter the expression and function of NET in DRG and CG neurons, and that is consistent with the findings that the NET mRNA expression is decreased in DRG neurons in the spinal nerve ligation rats. This suggests that the properties of sensory neurons can be modified by adrenergic and purinergic neurotransmitters.
Supported by Michigan State University
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2003 Neuroscience Meeting Planner. New Orleans, LA: Society for Neuroscience, 2003. Online.
Copyright © 2003-2026 Society for Neuroscience; all rights reserved. Permission to republish any abstract or part of any abstract in any form must be obtained in writing by SfN office prior to publication.
