Neuroscience 2000 Abstract
| Presentation Number: | 647.15 |
|---|---|
| Abstract Title: | Elimination of systemic physiologic confounds in pharmacologic MRI (PHMRI): a preliminary MRI-EEG study in the anesthesized rat at 7 tesla. |
| Authors: |
Kalisch, R.*1
; Elbel, G. K.1
; Gössl, C.1
; Czisch, M.1
; Auer, D. P.1
1Max-Planck-Inst Psychiatry, Munich, Germany |
| Primary Theme and Topics |
H. Other Systems of the CNS - 101. Brain metabolism and blood flow |
| Secondary Theme and Topics | J. Disorders of the Nervous System and Aging<br />- 147. Neuropsychiatric disorders: pharmacological agents |
| Session: |
647. Brain metabolism and blood flow II Poster |
| Presentation Time: | Wednesday, November 8, 2000 10:00 AM-11:00 AM |
| Location: | Hall G-J |
| Keywords: | ANXIETY, DIAZEPAM, BENZODIAZEPINE, BLOOD FLOW |
phMRI is a new tool for studying drug effects on brain activity. The BOLD (blood oxygenation level dependent) contrast is based on changes in blood oxygenation following changes in brain metabolism but is also influenced by systemic cardiovascular and respiratory variables. We evaluated if systemic effects on BOLD can be separated from specific cerebral drug effects. In 3 ventilated rats under isoflurane anesthesia temperature, resp. gases, blood pressure (BP), heart rate, EEG and ECG were measured during MR scans (7T, SE-EPI). Each animal received i.v. saline after 10 min, 50mg/kg of the anxiogenic pentylenetetrazole (PTZ) after 20 min and 1 mg/kg diazepam after 30 min. For data analysis the BOLD time courses were detrended with the CO2 and BP curves. The positive PTZ response became smaller after detrending (obviously, a concomitant BP rise artificially enhanced the BOLD increase). The consecutive diazepam-induced BOLD decrease emerged stronger after detrending (systemic influences tended to mask the cerebral drug effect). The separation of systemic and specific cerebral drug effects is esp. important for detecting subtle changes in brain activity induced by anxiogenic and anxiolytic substances.
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2000 Neuroscience Meeting Planner. New Orleans, LA: Society for Neuroscience, 2000. Online.
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