Neuroscience 2003 Abstract
| Presentation Number: | 580.7 |
|---|---|
| Abstract Title: | Regulation of the hemicholinium-3 sensitive CHO line transporter following electrical stimulation in the mouse striatum. |
| Authors: |
Gates, J.*1
; Clark, J. M.1
; Mbye, L.1
; Apparsundaram, S.1
1Dept. Anat. & Neurobiol, Univ. Kentucky Med. Ctr, Lexington, KY |
| Primary Theme and Topics |
Synaptic Transmission and Excitability - Transporters -- Monoamines, GABA, etc. |
| Secondary Theme and Topics | Synaptic Transmission and Excitability<br />- Neurotransmitters<br />-- Acetylcholine |
| Session: |
580. Transporters: Glycine, Other Poster |
| Presentation Time: | Tuesday, November 11, 2003 10:00 AM-11:00 AM |
| Location: | Morial Convention Center - Hall F-I, Board # D54 |
| Keywords: | ACETYLCHOLINE, UPTAKE, TRAFFICKING, PRESYNAPTIC |
In cholinergic neurons, the hemicholinium-3 (HC-3) sensitive, Na+/Cl--coupled, high affinity choline cotransporter (CHT) provides choline for acetylcholine (ACh) biosynthesis. The activity of CHT is tightly regulated by neuronal activity, and second messengers. Recent studies reveal that CHT is located in multiple compartments, including the plasma membrane and synaptic vesicles (Ferguson et al., Abstract, SFN 2002). We also have reported a change in the surface distribution of CHT proteins in response to protein kinase C (PKC) activation and protein phosphatase PP1/PP2A inhibition in the mouse striatum (Gates et al., Abstract, SFN 2002). In the present investigation, we examine the influence of electrical stimulation (square wave pulses, 50 Volts, 10 msec pulse duration) on CHT function in superfused mouse striatal slices. Crude striatal synaptosomes were prepared at the end of the stimulation period, and CHT-mediated choline uptake was measured using [3H] choline. Electrical stimulation at 5Hz for 30 min, 10 Hz for 2min and 10Hz for 5min produced significant reductions in CHT activity (5Hz, 30min = 50±13%; 10Hz, 2min = 49±12%; 10Hz, 5min = 45±4%). However, there was no significant change in CHT activity following electrical stimulation at 2 Hz for 2, 5 and 30 min. Sodium channel blocker, tetradotoxin(10µM) abolished the electrical stimulus (10Hz, 2min) evoked reduction in CHT function. These results indicate that electrical depolarization may alter CHT activity in a frequency-dependent manner. Ongoing experiments investigate changes in CHT surface distribution following electrical depolarization at different stimulation frequencies. These investigations may provide insight into presynaptic mechanisms involved in the control of function, surface expression and trafficking of CHT.
Supported by NIH P20RR15592
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2003 Neuroscience Meeting Planner. New Orleans, LA: Society for Neuroscience, 2003. Online.
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