Taste buds contain multiple cell types, 2 of which mediate transduction of specific taste qualities: Type III cells transduce sour while Type II cells transduce either sweet, or bitter or umami. In order to discern the degree of interaction between different cell types and specificity of connectivity with the afferent nerve fibers, we employed serial blockface scanning electron microscopy through 5 circumvallate mouse taste buds. Points of contact between Type II and III cells are rare, and lack morphologically identifiable synapses, suggesting that interaction between these cell types does not occur via synapses. Of the 127 nerve fibers that make synaptic contacts with taste cells in the sampling volume, about 70% (n=91) synapse with only one taste cell while 32 fibers synapse exclusively with multiple Type II cells or multiple Type III cells. Our data do not rule out multimodal fibers innervating Type II cells of separate taste qualities. Notably, 4 fibers (∼3%) synapse with both Type II and Type III cel...