At intermediate (‘mesopic’) light levels, rods and cones are both active and can contribute to vision. This presents a challenge to the retina, since the visual responses originating with rods and cones are distinct, yet their visual responses must be seamlessly combined. The current study aimed to establish how the circadian clock regulates rod and/or cone vision in these conditions, given the strong time-of-day change in the reliance on each photoreceptor. Visual responses were recorded in the retina and visual thalamus of anaesthetised male mice at distinct circadian time points, and the method of receptor silent substitution was used to selectively stimulate different photoreceptor types. With stimuli designed to only activate rods, responses in the mesopic range were highly rhythmic, and peaked in amplitude in the subjective night. This rhythm was abolished following intravitreal injection of the gap junction blocker MFA, consistent with a circadian variation in the strength of electrical coupling of ...