Neuroscience 2000 Abstract
| Presentation Number: | 577.14 |
|---|---|
| Abstract Title: | Distinct isoforms of tau aggregated in neurons and glial cells in brains of patients with sporadic tauopathies. |
| Authors: |
Arai, T.*1
; Ikeda, K.1
; Akiyama, H.1
; Shikamoto, Y.2
; Tsuchiya, K.3
; Yagishita, S.4
; Beach, T.5
; Rogers, J.5
; Schwab, C.6
; McGeer, P. L.6
1Tokyo Inst. of Psychiat., Tokyo, Japan 2Meiji Pharmaceutical Univ., Tokyo, Japan 3Tokyo Met. Matsuzawa Hosp., Tokyo, Japan 4Kanagawa Rehabilitation Center, Kanagawa, Japan 5Sun Health Research Inst., Sun City, AZ 6Univ. of British Columbia, Vancouver, Canada |
| Primary Theme and Topics |
J. Disorders of the Nervous System and Aging - 130. Degenerative disease: Alzheimer's-other |
| Secondary Theme and Topics | J. Disorders of the Nervous System and Aging<br />- 132. Degenerative disease: other |
| Session: |
577. Degenerative disease: Alzheimer's--other: tau III Poster |
| Presentation Time: | Tuesday, November 7, 2000 2:00 PM-3:00 PM |
| Location: | Hall G-J |
| Keywords: | ALZHEIMER, DEGENERATION, DEMENTIA, GLIA |
We investigated isoform composition of aggregated tau in the brains of Pick’s disease (PiD), corticobasal degeneration (CBD), progressive supranuclear palsy (PSP), argyrophilic grain disease (AGD) and ‘senile dementia of the tangle type (SDT)’ by immunoblot analysis of sarkosyl-insoluble fractions of brain homogenates. We also examined the adjacent brain tissues immunohistochemically with a rabbit antibody, Ex10, which specifically recognizes exon 10 of tau. The Ex10 recognizes tau isoforms with four microtubule-binding repeats (4Rtau) but not those with three microtubule-binding repeats (3Rtau). Sarkosyl-insoluble tau from the brains of patients with CBD and PSP consisted of 4Rtau. Insoluble tau from the brains of PiD, AGD, and SDT contained both 3Rtau and 4Rtau, where 3Rtau predominated over 4Rtau in PiD brains. In brain tissues of CBD and PSP, Ex10 immunostained all neuronal and glial structures such as pre-tangles, astrocytic plaques and oligodendroglial coiled bodies. In the PiD brains, astrocytic inclusions were also positive for 4Rtau. However, the majority, if not all, of both Pick bodies and oligodendroglial tau inclusions were negative for 4Rtau. Such results suggest that, in neurons and oligodendroglia, tau isoforms involved in the pathological processes differ between CBD/PSP and PiD, and are thus disease-specific. This contrasts with the astrocytic tau isoforms that accumulate similarly in these disorders.
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2000 Neuroscience Meeting Planner. New Orleans, LA: Society for Neuroscience, 2000. Online.
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