Neuroscience 2005 Abstract
| Presentation Number: | 491.9 |
|---|---|
| Abstract Title: | Initial characterization of a novel cannabinoid receptor in glial cells. |
| Authors: |
Reyes, F.*1
; Lauckner, J. E.2
; Xu, C.1
; Mackie, K.2,3
; Stella, N.1,4
1Pharmacology, Univ. of Washington, seattle, WA 2Physiology and Biophysics, Univ. of Washington, seattle, WA 3Anesthesiology, Univ. of Washington, seattle, WA 4Psychiatry and Behavioral Sciences, Univ. of Washington, seattle, WA |
| Primary Theme and Topics |
Neural Excitability, Synapses, and Glia: Cellular Mechanisms - G-Protein Linked Receptors -- Other |
| Session: |
491. Cannabinoid, Sigma, Histamine, and Melatonin Receptors Poster |
| Presentation Time: | Monday, November 14, 2005 1:00 PM-2:00 PM |
| Location: | Washington Convention Center - Hall A-C, Board # E35 |
| Keywords: | CANNABINOIDS, GLIA, PHOSPHOLIPASE, CALCIUM |
Several studies have demonstrated that cannabinoids may induce specific biological effects by interacting with an orphan G protein-coupled receptor. Recently, it has been suggested that GPR55 might constitute such a receptor. Indeed, we found that THC (5 µM) increased intracellular calcium in HEK293 cells expressing GPR55. GPR55 mRNA was present in spleen, microglial cells and astrocytes, as well as BV2 and N9 cells (two mouse microglia cell lines), suggesting its involvement in regulating neuroinflammation. On the other hand, GPR55 mRNA was not detected in OLN cells, an oligodendrocyte progenitor cell line, DBT, D1A and D3O, three astrocytic cell lines, suggesting that undifferentiated glial cells do not express this receptor subtype. Finally, we found that THC (5 µM) only modestly increased IP3 production in BV2 cells, suggesting that mechanisms in addition to phospholipase C may be involved in GPR55-mediated increases in intracellular calcium. Our data identify GPR55 as a bona fide cannabinoid receptor that increases intracellular calcium when engaged by THC and is expressed by differentiated glial cells.
Supported by Supported by NIDA & NINDS
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2005 Neuroscience Meeting Planner. Washington, DC: Society for Neuroscience, 2005. Online.
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