Neuroscience 2001 Abstract
| Presentation Number: | 524.22 |
|---|---|
| Abstract Title: | CANNABINOID RECEPTOR 1 INCREASES WITH SLEEP REBOUND. |
| Authors: |
Martinez-Vargas, M.*1
; Murillo-Rodriguez, E.1
; Gonzalez-Rivera , R.1
; Landa, A.2
; Velazquez-Moctezuma, J.3
; Prospero-Garcia, O.1
; Navarro, L.1
1Fisiologia, UNAM Fac Medicina, Mexico D.F., Mexico 2Microbiologia, UNAM Fac Medicina, Mexico D.F., Mexico 3UAM-I, Mexico D.F., Mexico |
| Primary Theme and Topics |
Autonomic, Limbic and Other Systems - Biological Rhythms and Sleep |
| Secondary Theme and Topics | Synaptic Transmission and Excitability<br />- Neurotransmitters<br />-- Cannabinoids |
| Session: |
524. Biological rhythms and sleep XII Poster |
| Presentation Time: | Tuesday, November 13, 2001 9:00 AM-10:00 AM |
| Location: | Exhibit Hall SS-3 |
| Keywords: | SLEEP DEPRIVATION, REM SLEEP |
Delta-9-THC, the most active of the psychoactive substances extracted from marijuana, binds to two types of cannabinoid receptors: CB1R and CB2R. The former has a preferential distribution in CNS and may be mediating the marijuana effects on sleep, which include increase in subjective sleepiness, alpha waves in the EEG and slow wave sleep. We previously reported that anandamide, an endogenous lipid that binds to CB1R increases SWS2 and Rapid Eye Movement Sleep (REMS). This study aims to analyze the effect of sleep deprivation and rebound on the CB1R expression in pons. Ten Wistar male rats were total sleep deprivated (TSD) by gentle manipulation for 24 hours. Five of them were allowed to rebound for two hours (TSD + R). They were sacrificed at the same hour that control rats (n=5). Fifteen rats were implanted with electrodes to analyze EEG and EMG. After surgery recover, ten of them, were REMS deprivated (REMSD) for 12 hours by analyzing their poligraphic records. Five of them were allowed to rebound for two hours (REMSD+R). Crude extracts of pons were analyzed by western blot using serum anti-CB1R. Results indicated that TSD or REMSD did not modified CB1R expression but it highly increased in pons of TSD+R and REMSD+R groups. These data suggest an involvement of CB1R in sleep regulation.
Supported by PAPPIT IIN233199 given to LN
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2001 Neuroscience Meeting Planner. San Diego, CA: Society for Neuroscience, 2001. Online.
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