Neuroscience 2001 Abstract
| Presentation Number: | 429.12 |
|---|---|
| Abstract Title: | The Difference of Tau Protein Isoforms Between White Matter and Cortex in Progressive Supranuclear Palsy and Corticobasal Degeneration. |
| Authors: |
Motoi, Y.*1
; Kato, E.1
; Arima, K.3
; Ikeda, K.2
; Mori, H.1
; Mizuno, Y.1
1Dept. of Neurology, Juntendo University School of Medicine, Tokyo, Japan 2Dept. of Ultrastructure and Immunohistochemistry, Tokyo Institute of Phychiatry, Tokyo, Japan 3Dept. of Laboratory Medicine, National Center Hospital for Mental, Nervous, and Muscular Disorders, National Center of Neurology and Psychiatry, Tokyo, Japan |
| Primary Theme and Topics |
Neurological and Psychiatric Conditions - Neurodegenerative Disorders -- Alzheimers Disease: Tau |
| Session: |
429. Neurodegenerative disorders: Alzheimer's disease: tau--neuropathology Poster |
| Presentation Time: | Monday, November 12, 2001 4:00 PM-5:00 PM |
| Location: | Exhibit Hall WW-47 |
| Keywords: | glia, tauopathy |
〔Objective.〕 In progressive supranuclear palsy (PSP) and corticobasal degeneration (CBD), phosphorylated tau accumulates in both nerve cells and glial cells. In PSP and CBD, the pathological tau contains only four repeat tau. In this study, we examined the difference of the tau protein isoforms of PSP and CBD between white matter and cortex.
〔Methods.〕 Sarcosyl-insoluble fraction was extracted from white matter and cortex in four cases of PSP and two of CBD following the protocol (Goedert et al, Neuron 1989 3(4):519-26). Insoluble tau was subjected to the western blot with a phosphorylation-dependent anti-tau antibody AT8 .
〔Results and discussion〕The pattern of the three bands of 64, 68 and 72KDa in PSP and CBD was different between cerebral cortex and white matter. When the relative amounts of these bands were compared between white matter and cortex, in white matter, 68KDa band was prominent when compared to 72KDa band (P<0.05). In cortex, tau deposits are both neruronal and glial, whereas in white matter, the cellular tau pathology is exclusively glial. Therfore pathological tau isoforms in glial cells may be different to those in neuronal cells.
〔Conclusions〕 Our results demonstrate that there is a difference in the composition of tau isoforms between white matter and cortex. It is likely that the pathological tau isoforms in glial cells may be different from those in neurons.
〔Methods.〕 Sarcosyl-insoluble fraction was extracted from white matter and cortex in four cases of PSP and two of CBD following the protocol (Goedert et al, Neuron 1989 3(4):519-26). Insoluble tau was subjected to the western blot with a phosphorylation-dependent anti-tau antibody AT8 .
〔Results and discussion〕The pattern of the three bands of 64, 68 and 72KDa in PSP and CBD was different between cerebral cortex and white matter. When the relative amounts of these bands were compared between white matter and cortex, in white matter, 68KDa band was prominent when compared to 72KDa band (P<0.05). In cortex, tau deposits are both neruronal and glial, whereas in white matter, the cellular tau pathology is exclusively glial. Therfore pathological tau isoforms in glial cells may be different to those in neuronal cells.
〔Conclusions〕 Our results demonstrate that there is a difference in the composition of tau isoforms between white matter and cortex. It is likely that the pathological tau isoforms in glial cells may be different from those in neurons.
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2001 Neuroscience Meeting Planner. San Diego, CA: Society for Neuroscience, 2001. Online.
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