Neuroscience 2001 Abstract
| Presentation Number: | 291.5 |
|---|---|
| Abstract Title: | CANNABINOID MODULATION OF GLUTAMATE RELEASE IN RAT STRIATUM. |
| Authors: |
Brown, T. M.*1
; Prince, R. J.1
; Brotchie, J. M.1
1Neuroscience, University of Manchester, Manchester, United Kingdom |
| Primary Theme and Topics |
Motor Systems - Basal Ganglia -- Transmitters and receptors |
| Session: |
291. Basal ganglia: transmitters and receptors II Poster |
| Presentation Time: | Monday, November 12, 2001 8:00 AM-9:00 AM |
| Location: | Exhibit Hall GG-12 |
| Keywords: | CANNABINOIDS |
The active constituents of cannabis act through the G-protein coupled receptors CB1 and CB2. CB1 receptors in the striatum mediate profound motor effects when cannabinoids are administered to rodents. Electrophysiological studies suggest CB1 activation can reduce striatal glutamate release, by presynaptic inhibition. We investigated the effects of cannabinoids on glutamate release directly, using superfused striatal slices taken from Sprague Dawley rats. Slices were preloaded with [3H]-glutamic acid and released radioactivity was measured by liquid scintillation counting. Application of a depolarising K+ pulse (40 mM) induced the release of glutamate from striatal slices in a Ca2+-dependent manner. Application of 10μM Δ9-tetrahydrocannabinol (Δ9-THC), a CB1 receptor agonist, significantly reduced (by 44%) the depolarisation-evoked release of [3H]-glutamic acid (P<0.01). The CB1 receptor antagonist SR141716A (3μM) did not effect the depolarisation-evoked release of [3H]-glutamic acid, but blocked the effects of 10μM Δ9-THC. These data indicate CB1 activation inhibits glutamate release in the rat striatum and that, in this preparation, endogenous cannabinoids do not contribute to the regulation of striatal glutamate release. These findings support the previous suggestion that cannabinoids may have potential in the treatment of basal ganglia disorders.
Supported by BBSRC
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2001 Neuroscience Meeting Planner. San Diego, CA: Society for Neuroscience, 2001. Online.
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