Neuroscience 2001 Abstract
| Presentation Number: | 256.1 |
|---|---|
| Abstract Title: | ROLE OF ENDOCANNABINOIDS IN FOOD INTAKE AND DEVELOPMENT AFTER BIRTH: CB1 RECEPTOR SPECIFICITY. |
| Authors: |
Fride, E. C.*1
; Hanus, L.2
; Mechoulam, R.2
1Dept Behavioral Sciences, College of Judea & Samaria, Ariel, Israel 2Medicinal Chemistry and Natural Products, Hebrew University of Jerusalem, Jerusalem, Israel |
| Primary Theme and Topics |
Development - Epigenetic Factors -- Nutrition and prenatal factors |
| Secondary Theme and Topics | Cognition and Behavior<br />- Development of Behavior |
| Session: |
256. Epigenetic factors: nutrition and perinatal factors Poster |
| Presentation Time: | Monday, November 12, 2001 8:00 AM-9:00 AM |
| Location: | Exhibit Hall C-29 |
| Keywords: | cannabinoids, feeding, neonatal, antagonist |
Cannabis enhances appetite. We have demonstrated a critical role for the endocannabinoid system in milk ingestion and survival of newborn mice by blocking the cannabinoid (CB1) receptor (with SR141817A). Co-administration of tetrahydrocannabinol (THC) reversed these effects.
In the present experiments, we further investigated the specificity of the endocannabinoid-CB1 receptor system as a mediator of SR141716A-induced pup mortality. Mouse pups (Sabra) were injected within 24 hr after birth. (1) SR141716A-induced pup mortality was dose dependent (5-20 mg/kg). (2) The endocannabinoid 2-arachidonoyl glycerol (2-AG) did not reverse the effect of SR141716A. However, 2-AG in combination with its entourage (2-palmitoyl glycerol and 2-linoleoyl glycerol) attenuated the effect of SR141716A. (3) Cannabidiol (CBD) a non psychotropic cannabinoid which does not bind to the CB1 receptor, did not reverse the growth-arresting effects of the CB1 antagonist. (4) The CB2 (peripheral CB receptor) antagonist SR144528 did not affect pup growth.
(5) Lysophosphatidic acid (LPA) has growth factor like properties and is structurally similar to 2-AG. LPA and CB1 receptors display substantial homology. Do LPA and endocannabinoids interact to affect newborn development? LPA attenuated SR141716A-induced growth stunting. Standard LPA showed no significant binding to the CB1 receptor.
We conclude that SR141716A-induced inhibition of neonatal food intake and growth is mainly mediated by CB1 receptor blockade. However, possible cross-talk between LPA and the endocannabinoid systems deserves further study.
.
In the present experiments, we further investigated the specificity of the endocannabinoid-CB1 receptor system as a mediator of SR141716A-induced pup mortality. Mouse pups (Sabra) were injected within 24 hr after birth. (1) SR141716A-induced pup mortality was dose dependent (5-20 mg/kg). (2) The endocannabinoid 2-arachidonoyl glycerol (2-AG) did not reverse the effect of SR141716A. However, 2-AG in combination with its entourage (2-palmitoyl glycerol and 2-linoleoyl glycerol) attenuated the effect of SR141716A. (3) Cannabidiol (CBD) a non psychotropic cannabinoid which does not bind to the CB1 receptor, did not reverse the growth-arresting effects of the CB1 antagonist. (4) The CB2 (peripheral CB receptor) antagonist SR144528 did not affect pup growth.
(5) Lysophosphatidic acid (LPA) has growth factor like properties and is structurally similar to 2-AG. LPA and CB1 receptors display substantial homology. Do LPA and endocannabinoids interact to affect newborn development? LPA attenuated SR141716A-induced growth stunting. Standard LPA showed no significant binding to the CB1 receptor.
We conclude that SR141716A-induced inhibition of neonatal food intake and growth is mainly mediated by CB1 receptor blockade. However, possible cross-talk between LPA and the endocannabinoid systems deserves further study.
.
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2001 Neuroscience Meeting Planner. San Diego, CA: Society for Neuroscience, 2001. Online.
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