Neuroscience 2000 Abstract
| Presentation Number: | 815.11 |
|---|---|
| Abstract Title: | Transient upregulation of NCAM expression after acute but not chronic cannabinoid treatment: validation of large scale cDNA array screening. |
| Authors: |
Grigorenko, E. V.*1
; Bundey, S.1
; Deadwyler, S. A.1
1Department of Physiology and Pharmacology, Wake Forest University, School of Medicine, Winston-Salem, NC |
| Primary Theme and Topics |
D. Neurotransmitters, Modulators, Transporters, and Receptors - 54. Cannabinoids |
| Secondary Theme and Topics | J. Disorders of the Nervous System and Aging<br />- 146. Drugs of abuse: opioids and others |
| Session: |
815. Cannabinoids: receptor structure and localization Poster |
| Presentation Time: | Thursday, November 9, 2000 10:00 AM-11:00 AM |
| Location: | Hall G-J |
| Keywords: |
Chronic administration of cannabinoids has been shown to result in a high degree of tolerance in which physiological and behavioral functions revert back to near normal (control) levels but important second messenger pathways remain altered (Deadwyler et al., 1995; Sim et al., 1996). Large scale cDNA microarray screenings revealed an increased expression of NCAM in regions with high CB1 receptor densities-hippocampus and cerebellum, after a single high dose of Δ9-tetrahydrocannabinol (Δ9-THC, 10 mg/kg). To confirm these findings of changes in NCAM expression, in situ hybridization technique was applied for analysis of NCAM expression in different hippocampal and cerebellar subregions. In all three tested groups, vehicle-, Δ9-THC-and SR171416A+ Δ9-THC -treated animals, NCAM probe revealed distinct staining of pyramidal neurons and granule cells of the dentate gyrus. Compared to the vehicle-treated control, a significant increase in the hybridization signal of NCAM -positive cells was observed in the granular cell layer (by 45%, p<0.01) and in Ammon's horn region (by 42%, p<0.01) after a single injection of Δ9-THC. This increase was partially abolished by co-injection of cannabinoid receptor antagonist, SR171416A. After treatment for 7 days this increase in NCAM was no longer apparent. Application of the cDNA large scale microarray screen with in situ hybridization provided valuable information on potential new linkages between the dynamics of cannabinoid receptor activation and transient changes in NCAM expression which has been shown to be critical for plasticity in hippocampal neurons.
Supported by NIDA grants DA03502, DA07625 & DA00119 to SAD; AG00743 to EVG.
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2000 Neuroscience Meeting Planner. New Orleans, LA: Society for Neuroscience, 2000. Online.
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