Neuroscience 2002 Abstract
| Presentation Number: | 830.1 |
|---|---|
| Abstract Title: | Agonist-mediated increase of intracellular cAMP through human CB1R in the presence of pertussis toxin. |
| Authors: |
Chen, J.*1
; Foor, F.1
; Van der Ploeg, L. H. T.1
; Fong, T. M.1
; Shen, C.1
1Merck, Rahway, NJ |
| Primary Theme and Topics |
Synaptic Transmission and Excitability - G-Protein Linked Receptors -- Other |
| Session: |
830. G-protein linked receptors II Poster |
| Presentation Time: | Thursday, November 7, 2002 8:00 AM-9:00 AM |
| Location: | Hall A2-B3 C-36 |
| Keywords: | cannabinoid, receptor, signaling, G protein |
The cannabinoid CB1 receptor (CB1R) is an abundantly expressed G-protein coupled receptor in the brain. THC (tetrahydrocannabinol), the active ingredient in marijuana, is an agonist at CB1R. In addition to THC, CP55940 is a commonly used agonist for CB1R. The agonist-receptor interactions result in activation of G proteins, particularly those of the Gi/o family, to inhibit the activity of adenylyl cyclase and regulate ion channels. In this study, CHO cell lines stably expressing CB1R (CHO-CB1R) were created. Consistent with previous observation and the expectation of Gi-coupling, CP55940 decreased the forskolin-stimulated cAMP accumulation in CHO-CB1R cells. In the presence of PTX, however, CP55940 augmented the forskolin-stimulated response, probably through Gs. The potential of CB1R activating multiple G proteins or multiple signaling pathways will be discussed.
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2002 Neuroscience Meeting Planner. Orlando, FL: Society for Neuroscience, 2002. Online.
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