Neuroscience 2001 Abstract
| Presentation Number: | 805.12 |
|---|---|
| Abstract Title: | MICROARRAY ANALYSIS OF CEREBRAL GENE EXPRESSION CHANGES FOLLOWING CB1 CANNABINOID RECEPTOR ACTIVATION. |
| Authors: |
Parmentier-Batteur, S.*1
; Eshoo, M.1
; Felkey, K.1
; Jin, K.1
; Greenberg, D. A.1
1Buck Center for Age Research, Novato, CA |
| Primary Theme and Topics |
Synaptic Transmission and Excitability - Neurotransmitters -- Cannabinoids |
| Secondary Theme and Topics | Synaptic Transmission and Excitability<br />- Intracellular Signalling Pathways<br />-- Other |
| Session: |
805. Neurotransmitters: cannabinoids Poster |
| Presentation Time: | Wednesday, November 14, 2001 4:00 PM-5:00 PM |
| Location: | Exhibit Hall D-28 |
| Keywords: | gene expression, mice, microarray, cannabinoids |
Recent experimental evidence suggests oppositely directed actions of cannabinoids, promoting either cell survival in models of stroke, parkinson’s disease and multiple sclerosis, or cell death in models of malignant glioma. However the mechanisms through which these compounds control the cell survival/death decision remain to be established. To identify potential mediators of cannabinoid effects, we employed cDNA microarrays to assess changes in gene expression levels following exposure to two different CB1 receptor agonists in mice. A total of 11,000 cDNA clones were selected from a mouse brain cDNA library, amplified by PCR, and arrayed at high density to investigate differential gene expression profiles following acute (12-h) exposure to either Δ9-tetrahydrocannabinol (Δ9-THC) (10 mg/kg i.p.) or WIN 55,212-2 (1 mg/kg i.p.). cDNA microarrays for each treatment were performed in triplicate. Results revealed a total of 75 genes altered by Δ9-THC and 36 genes altered by WIN 55,212-2; of these, 12 were altered by both treatments. Genes whose expression was modified by both CB1 agonists included genes involved in either cell death (down-regulation of MAP2, phosphatase A2 and S-adenosylmethionine synthase; up-regulation of p53 associated target) or cell survival and proliferation (up-regulation of CBP/p300 and procollagen IIIa). Genes altered in common by Δ9-THC and WIN 55,212-2 are likely to be involved in CB1 receptor-mediated signaling, whereas genes uniquely affected by one or the other drug may reflect non-CB1 effects. The significance of these changes in cerebral gene expression for cannabinoid system function remained to be determined.
Supported by NIH NS39912
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2001 Neuroscience Meeting Planner. San Diego, CA: Society for Neuroscience, 2001. Online.
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