Neuroscience 2000 Abstract
| Presentation Number: | 789.3 |
|---|---|
| Abstract Title: | Acute and chronic central effects of cannabinoids in mice are independent of the transcription factor Krox-24. |
| Authors: |
Tzavara, E. T.*1
; Monory, K.1
; Garel, S.2
; Topilko, P.2
; Borsodi, A.3
; Charnay, P.2
; Hanoune, J.1
1U-99, INSERM, Creteil, France 2U-368, INSERM, Paris, France 3Inst. of Biochemistry, Biological Research Center, Szeged, Hungary |
| Primary Theme and Topics |
D. Neurotransmitters, Modulators, Transporters, and Receptors - 60. Signal transduction: gene expression and plasticity |
| Secondary Theme and Topics | J. Disorders of the Nervous System and Aging<br />- 146. Drugs of abuse: opioids and others |
| Session: |
789. Signal transduction: gene expression and plasticity Slide |
| Presentation Time: | Thursday, November 9, 2000 8:30 AM-8:45 AM |
| Location: | Room 383 |
| Keywords: | cerebellum, withdrawal, THC, adenylate cyclase |
Krox-24 (also known as Egr-1, NGF1-A, Zif-268, and tis-8) is an immediate early gene encoding a zinc-finger transcription factor implicated in several adaptive responses, and induction by cannabinoids has been reported in vivo and in different cell lines. We used mice targeted in the Krox-24 gene to specifically dissect the role of this transcription factor in the acute and chronic central actions of cannabinoids. We report here on the ability of cannabinoids to activate G-proteins and to inhibit adenylyl cyclase activity in in vitro functional assays, and to elicit behavioral responses in wild-type and mutant mice. Analgesia and locomotor activity were assessed after a single administration of D9-tetrahydrocannabinol (D9-THC). After chronic D9-THC administration, D9-THC withdrawal was precipitated by the use of the selective antagonist of the cannabinoid receptor 1, SR141716A, and the behavioral parameters and the biochemical correlates of abstinence were evaluated. Despite minor differences in the various parameters studied, the overall acute and chronic effects of cannabinoids were similar in the mutant as compared to wild-type mice. We conclude that Krox-24 is not a limiting factor for cannabinoid actions.
Supported by This work was supported by the INSERM, the Ministère de la Recherche et de la Technologie (MILDT grand 97.002), by Sanofi-Synthelabo who also provided SR141716A, and by the NATO Fellowships Program.
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2000 Neuroscience Meeting Planner. New Orleans, LA: Society for Neuroscience, 2000. Online.
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