Neuroscience 2005 Abstract
| Presentation Number: | 675.7 |
|---|---|
| Abstract Title: | Localization of the cannabinoid CB1 receptor in monkey and human prefrontal cortex. |
| Authors: |
Eggan, S. M.*1,2
; Lewis, D. A.1,3
1Dept Neurosci, Univ. of Pittsburgh, Pittsburgh, PA 2Center for the Neural Basis of Cognition, Univ. of Pittsburgh, Pittsburgh, PA 3Dept Psych, Univ. of Pittsburgh, Pittsburgh, PA |
| Primary Theme and Topics |
Disorders of the Nervous System - Cognitive, Emotional and Behavioral State Disorders -- Schizophrenia: Pathology |
| Secondary Theme and Topics | Disorders of the Nervous System<br />- Cognitive, Emotional and Behavioral State Disorders<br />-- Schizophrenia: Animal models |
| Session: |
675. Pathology of Schizophrenia: Genes and Expression II Poster |
| Presentation Time: | Tuesday, November 15, 2005 10:00 AM-11:00 AM |
| Location: | Washington Convention Center - Hall A-C, Board # TT46 |
| Keywords: | GABA, INTERNEURON, IMMUNOCYTOCHEMISTRY, SCHIZOPHRENIA |
Delta-9-tetrahydrocannabinol (Δ9-THC) has profound effects on cognitive functions subserved by the prefrontal cortex (PFC), and exposure to THC has been associated with the appearance or exacerbation of the clinical features of schizophrenia. These actions appear to be mediated via the CB1 receptor, the only cannabinoid receptor expressed in the brain. We therefore investigated the immunocytochemical localization of the CB1 receptor in the macaque monkey and human PFC using antibodies that specifically recognize the C-terminus of the CB1 receptor. In monkeys, intense CB1-immunoreactivity (CB1-IR) was observed primarily in axons and boutons. Compared to most other regions, such as the primary visual cortex (PVC), the overall density of CB1-IR axons was much higher in the PFC. The PFC also exhibited a dense band of CB1-IR axons in layer 4, whereas in PVC layer 4 contained the lowest density of CB1-IR axons. The same regional and laminar distributions of CB1-IR were also present in the human cortex. CB1-IR axons appeared more varicose in humans, but this difference appeared to be due to postmortem interval (PMI) as similar morphological differences were present in monkey tissue with varying PMIs. Electron microscopy studies of perfused monkey PFC revealed that CB1-IR is predominantly found in axon terminals that exclusively form symmetric synapses. Many of the observed positive terminals were found to make inputs onto cell bodies and proximal apical dendrites, characteristic of peri-somatic appositions made by basket interneurons. When CB1-IR was observed in cell bodies, they had the characteristic features of GABA neurons such as invaginated nuclei. The high density, distinctive laminar distribution, and localization to inhibitory terminals of CB1 receptors in the primate PFC suggests that the CB1 receptor may play a critical role in the circuitry that subserves cognitive functions such as those that are disturbed in schizophrenia.
Supported by MH51234 and MH43784
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2005 Neuroscience Meeting Planner. Washington, DC: Society for Neuroscience, 2005. Online.
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