Neuroscience 2001 Abstract
| Presentation Number: | 668.16 |
|---|---|
| Abstract Title: | CANNABINOID CB1 RECEPTORS WITHIN IN THE PREFRONTAL CORTEX AND STRIATUM CONTROL DOPAMINE AND ACETYLCHOLINE EFFLUX. |
| Authors: |
Verrico, C. D.*1
; Jentsch, J. D.2
; Dazzi, L.2
; Roth, R. H.1,2
1Pharmacology, Yale University, New Haven, CT 2Psychiatry, Yale University, New Haven, CT |
| Primary Theme and Topics |
Neurological and Psychiatric Conditions - Addiction and Drugs of Abuse -- Opioids and others |
| Secondary Theme and Topics | Synaptic Transmission and Excitability<br />- Neurotransmitters<br />-- Cannabinoids |
| Session: |
668. Addiction and drugs of abuse: opioids and others VI Poster |
| Presentation Time: | Tuesday, November 13, 2001 4:00 PM-5:00 PM |
| Location: | Exhibit Hall AAA-45 |
| Keywords: | cannabinoids, dopamine, acetycholine |
Acute administration of the cannabinoid CB1 receptor agonists, delta-9-tetrahydrocannabinol (THC) or WIN 55,212-2 (WIN) affects several neurotransmitter systems, including the dopamine (DA) and acetylcholine (ACh) innervations of the prefrontal cortex (PFC). In the current studies, we examined the effects of local application of cannabinoid CB1 receptor agonist(s)/antagonist via reverse dialysis on DA and ACh release in freely moving animals using in vivo microdialysis.
The infusion of THC (10 –5-10 –3 M) into the striatum produced a dose-dependent decrease in dopamine release with a maximal inhibitory effect of 50 % at a concentration of 10 –3 M but had no significant effect on acetylcholine release. Intra-striatal infusion of SR141716A (SR) (10 –5-10 –3 M), a selective CB1 receptor antagonist, was found to have no effect on transmitter release. We also tested the actions of these CB1 receptor agents infused into the PFC. THC was without effect on dopamine and acetylcholine release. However, SR was found to increase dopamine release with a maximal increase of 250% at the 10-4M concentration. Conversely, SR was found to have a maximal inhibitory effect on cortical acetylcholine release of 65% with the10 –3M concentration.
These studies demonstrate that cannabinoid receptors differentially regulate cholinergic and dopaminergic transmission in the PFC and striatum. Moreover, these data show that cholinergic and dopaminergic systems in PFC and striatum are to some extent independently regulated. Finally, the endogenous cannabinoid system is implicated in the regulation of these neurotransmitter systems.
The infusion of THC (10 –5-10 –3 M) into the striatum produced a dose-dependent decrease in dopamine release with a maximal inhibitory effect of 50 % at a concentration of 10 –3 M but had no significant effect on acetylcholine release. Intra-striatal infusion of SR141716A (SR) (10 –5-10 –3 M), a selective CB1 receptor antagonist, was found to have no effect on transmitter release. We also tested the actions of these CB1 receptor agents infused into the PFC. THC was without effect on dopamine and acetylcholine release. However, SR was found to increase dopamine release with a maximal increase of 250% at the 10-4M concentration. Conversely, SR was found to have a maximal inhibitory effect on cortical acetylcholine release of 65% with the10 –3M concentration.
These studies demonstrate that cannabinoid receptors differentially regulate cholinergic and dopaminergic transmission in the PFC and striatum. Moreover, these data show that cholinergic and dopaminergic systems in PFC and striatum are to some extent independently regulated. Finally, the endogenous cannabinoid system is implicated in the regulation of these neurotransmitter systems.
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2001 Neuroscience Meeting Planner. San Diego, CA: Society for Neuroscience, 2001. Online.
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