Neuroscience 2001 Abstract
| Presentation Number: | 668.15 |
|---|---|
| Abstract Title: | ULTRASTRUCTURAL ANALYSIS OF THE CANNABINOID RECEPTOR (CB1) IN THE RAT NORADRENERGIC LOCUS COERULEUS (LC) IN RAT BRAIN. |
| Authors: |
Oropeza, V. C.*1
; Peoples, J. F.1
; Mackie, K.2
; Van Bockstaele, E. J.1
1Dev Biol Teratol, Thomas Jefferson Univ, Philadelphia, PA 2Dept. of Anesthesiology, University of Washington, Seattle, WA |
| Primary Theme and Topics |
Neurological and Psychiatric Conditions - Addiction and Drugs of Abuse -- Opioids and others |
| Secondary Theme and Topics | Synaptic Transmission and Excitability<br />- Neurotransmitters<br />-- Catecholamines |
| Session: |
668. Addiction and drugs of abuse: opioids and others VI Poster |
| Presentation Time: | Tuesday, November 13, 2001 3:00 PM-4:00 PM |
| Location: | Exhibit Hall AAA-45 |
| Keywords: | norepinephrine, electron microscopy, glia, cannabis |
Several lines of evidence suggest direct actions of delta 9-tetrahydrocannabinol (THC), the main psychoactive constituent of Cannabis sativa, in modulating brain noradrenergic neurons. Noradrenergic neurons have been shown to play an important role in the manifestation of catalepsy induced by THC (Kataoka et al., Neuropharm. 26:55-60, 1987). In addition, cannabinoids activate descending noradrenergic neurons resulting in antinociception via the stimulation of spinal alpha 2-adrenoceptors (Lichtman and Martin, Brain Res. 559:309-314, 1991). Finally, exposure of pregnant rats to THC affects the gene expression and activity of tyrosine hydroxylase (TH) in the brains of their offspring (Bonnin et al., J Mol Neurosci, 7:291-308, 1996). In the present study, we examined the ultrastructural distribution of CB1 using immunoperoxidase or immunogold-silver labeling in the LC of adult male Sprague Dawley rats. Subsequently, we combined immunocytochemical detection of an antibody directed against the CB1 receptor and antibodies directed against TH or S100b, a marker for glial cells, to examine cellular targets of CB1 ligands with respect to noradrenergic neurons or glial cells in the LC. Using confocal fluorescence microscopy, CB1-immunoreactivity was prominently distributed within the LC area and was associated with both TH- and S100b-labeled cells. At the ultrastructural level, CB1 was distributed in TH labeled dendrites, glial processes and within axon terminals. These data indicate multiple cellular targets for CB1 ligands in the LC region.
Supported by NIDA DA09082, AHA 96002240.
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2001 Neuroscience Meeting Planner. San Diego, CA: Society for Neuroscience, 2001. Online.
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