Neuroscience 2001 Abstract
| Presentation Number: | 668.8 |
|---|---|
| Abstract Title: | ACTIVATION OF CANNABINOID RECEPTORS CAUSES REGION-SPECIFIC INCREASES IN RGS4 mRNA LEVELS IN BRAIN. |
| Authors: |
Gold, S. J.*1
; Pudiak, C. M.1
; Sim-Selley, L. J.2
; Nestler, E. J.1
; Martin , B. R.2
; Delley, D. E.2
1Psychiatry, UT Southwestern Med Cntr, Dallas, TX 2Pharm & Tox, Virginia Commonwealth Univ, Richmond, VA |
| Primary Theme and Topics |
Neurological and Psychiatric Conditions - Addiction and Drugs of Abuse -- Opioids and others |
| Secondary Theme and Topics | Synaptic Transmission and Excitability<br />- G-Protein Linked Receptors<br />-- Other |
| Session: |
668. Addiction and drugs of abuse: opioids and others VI Poster |
| Presentation Time: | Tuesday, November 13, 2001 4:00 PM-5:00 PM |
| Location: | Exhibit Hall AAA-45 |
| Keywords: | marijuana, mouse, anandamide, g protein signaling |
Recent evidence suggests that endogenous cannabinoids in the CNS inhibit neuronal activity and neurotransmitter release. These effects are mediated by Gi/o coupled CB1 receptors, which are abundantly expressed in cerebellum, hippocampus, cortex and basal ganglia. Additional evidence suggests that CB1 receptor signaling is negatively modulated in a use-dependent fashion. The novel regulators of G protein signaling (RGS) proteins have potent GTPase activating effects at Gi/o subunits and thus could subserve negative feedback of CB1R signaling. We are using several lines of investigation to test this hypothesis. Six hr after an acute injection of THC, or 24 hr after a 15 d regimen of daily THC injections, mice were killed and processed for in situ hybridization to RGS4 mRNA in sections of prefrontal cortex, striatum, and cerebellum. RGS4 mRNA levels were increased in entorhinal cortex and hippocampus with a trend towards increased expression in striatum, but not in thalamus, 6 hr after an acute injection of THC. In contrast, no significant effects were seen 24 hr after the chronic course of THC administration. Studies are ongoing to further examine RGS mRNA expression in mice treated both acutely and chronically with delta9-THC. These studies will help to elucidate the region- and possibly subtype- specific regulation of CB1 receptor signaling by RGS proteins in brain.
Supported by NIDA (DA-05274, DA-00287, DA-10770) and NARSAD
Sample Citation:
[Authors]. [Abstract Title]. Program No. XXX.XX. 2001 Neuroscience Meeting Planner. San Diego, CA: Society for Neuroscience, 2001. Online.
Copyright © 2001-2025 Society for Neuroscience; all rights reserved. Permission to republish any abstract or part of any abstract in any form must be obtained in writing by SfN office prior to publication.
