| Presentation Number: |
245.3 |
| Abstract Title: |
Functional electrical stimulation replenishes the neural progenitor pool in the adult CNS after spinal cord injury. |
| Authors: |
Becker, D.*1;
Grill, W. M.3;
McDonald, J. W.1,2
1Dept. of Neurol., Washington Univ, St Louis, MO
2SCI Restorative Treatment and Res. Ctr., Washington Univ, St Louis, MO
3OH, 4455 Duncan, 63108,
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| Primary Theme and Topics |
Development
- Transplantation and Regeneration
-- Regeneration: CNS
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| Secondary Theme and Topics |
Development
- Neurogenesis and Gliogenesis
-- Neural stem cells
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| Session: |
245. CNS Regeneration: Spinal Cord
Poster |
| Presentation Time: |
Sunday November 9, 2003 3:00 PM-4:00 PM |
| Location: |
Convention Center Exhibit Hall, Poster Board A59 |
| Keywords: |
activity, functional electrical stimulation, stem cells, regeneration
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The goal of these studies was to determine whether functional electrical stimulation (FES) can promote regeneration following chronic spinal cord injury. FES has traditionally focused on restoring function in conditions of central nervous system (CNS) injury where voluntary control has been lost consequent to injury. Studies of CNS development suggest that patterned neural activity might also be important for regeneration in conditions where neural activity is reduced such as the case in the spinal cord below the injury level. As index of regeneration we examined birth and survival of neural progenitors as well as differentiation in the chronically injured spinal cord following FES-induced stepping-like hindlimb movement. We implanted FES electrodes bilaterally into the hindlimbs of rats that three weeks earlier had received a complete suction ablation SCI at level T9, and stimulated half the rats for three hours per day for 12 days. Following a five-day Bromodeoxyuridine (BrdU) pulse labeling that began at day 7 of stimulation half of the animals were sacrificed. For an additional 7 days, the rest of the remaining rats continued the FES protocol and stimulation. FES induced a significant increase (60-80%) in cell birth selectively in the lumbar cord. In contrast, no difference was observed between groups at levels above the injury. The newly born cells mainly expressed markers of tripotential and glial progenitors, astrocytes, and oligodendrocytes. The effect of FES persists when examined after 7 days. The majority of progenitors differentiate into glial cells. However, about 50% remain undifferentiated after 7 days. This report represents the first demonstration that FES can enhance cellular regeneration in the injured adult CNS.
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| Supported by NIH grants NS37927, NS40520, NS36265. |
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| Sample Citation: |
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[Authors]. [Abstract Title]. Program No. XXX.XX. 2003 Neuroscience Meeting Planner. New Orleans, LA: Society for Neuroscience, 2003. Online.
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| Copyright © 2003-2010 Society for Neuroscience; all rights reserved. Permission to republish any abstract or part of any abstract in any form must be obtained in writing by SfN office prior to publication.
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